Electronic setup for fluorescence emission measurements and long-time constant-temperature maintenance of Single-Walled Carbon Nano-Tubes in water solutions

In our previous research we have observed that the fluorescence emission from water solutions of Single-Walled Carbon Nano-Tubes (SWCNT), excited by a laser with a wavelength of 830nm, diminishes with the time. We have already proved that such a fading is a function of the storage time and the storage temperature. In order to study the emission of the SWCNT as a function of these two parameters we have designed and realized a special measurement compartment with a cuvette holder where the SWCNT solutions can be measured and stored at a fixed constant temperature for periods of time as long as several weeks. To maintain the measurement setup under a constant temperature we have designed special experimental setup based on two Peltier cells with electronic temperature control

Norms and the meaning of omissive enabling conditions

People often reason about omissions. One line of research shows that people can distinguish between the semantics of omissive causes and omissive enabling conditions: for instance, not flunking out of college enabled you (but didn’t cause you) to graduate. Another line of work shows that people rely on the normative status of omissive events in inferring their causal role: if the outcome came about because the omission violated some norm, reasoners are more likely to select that omission as a cause. We designed a novel paradigm that tests how norms interact with the semantics of omissive enabling conditions. The paradigm concerns the circuitry of a mechanical device that plays music. Two experiments used the paradigm to stipulate norms and present a distinct set of possibilities to participants. Participants chose which causal verb best described the operations of the machine. The studies revealed that participants’ responses are best predicted by their tendency to consider the semantics of omissive relations. In contrast, norms had little to no effect in participants’ responses. We conclude by marshaling the evidence and considering what role norms may play in people’s understanding of omissions

Cortical granule exocytosis is mediated by alpha-SNAP and N-Ethilmaleimide sensitive factor in mouse eggs

Cortical granule exocytosis (CGE), also known as cortical reaction, is a calciumregulated secretion that represents a membrane fusion process during meiotic cell division of eggs. The molecular mechanism of membrane fusion during CGE is still poorly understood and is thought to be mediated by the SNARE pathway;nevertheless, it is unkown if SNAP (acronym for soluble NSF attachment protein) and NSF (acronym for N-ethilmaleimide sensitive factor), two key proteins in the SNARE pathway, mediate CGE in any egg model. In this paper, we documented the gene expression of α-SNAP, γ-SNAP and NSF in mouse oocytes. Western blot analysis showed that the expression of these proteins maintains a similar level during oocyte maturation and egg activation. Their localization was mainly observed at the cortical region of eggs, which is enriched in cortical granules. To evaluate the function of these proteins in CGE we set up a functional assay based on the quantification of cortical granules. Endogenous α-SNAP and NSF proteins were perturbed by microinjection of recombinant proteins or antibodies prior to CGE activation. The microinjection of wild type α-SNAP and the negative mutant of α-SNAP L294A in eggs inhibited CGE stimulated by strontium. NEM, an irreversibly inhibitor of NSF, and the microinjection ofthe negative mutant NSF D1EQ inhibited cortical reaction. The microinjection of anti-α-SNAP and anti-NSF antibodies was able to abolish CGE in activated eggs. The microinjection of anti-γ SNAP antibody had no effect on CGE. Our findings indicate, for the first time in any oocyte model, that α-SNAP, γ-SNAP, and NSF are expressed in mouse oocytes. We demonstrate that α-SNAP and NSF have an active role in CGE and propose a working model.Fil: de Paola, Maria Matilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo; ArgentinaFil: Bello, Oscar Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. University of Yale; Estados UnidosFil: Michaut, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentin

Experimental setup for light-to-heat NIR conversion measurements of gold nano-particles\u2019 solutions

In recent years, there is a constantly increasing interest in the application of nanoparticles for cancer diagnosis and cancer therapy. In this respect, the most promising nano-objects at present are the gold nanoparticles. A very convenient and powerful property of these objects is their ability to increase their temperature under electro-magnetic irradiation with certain wavelength. In our research we have directed our efforts toward particular nano-objects specifically sensitive to electromagnetic radiation in the near-infrared region (NIR). In order to study the photothermic properties of the solutions of gold nanoparticles in the NIR we constructed a specific electronic setup consisting of a laser system with interchangeable laser diodes with different wavelength NIR light, a thermally-insulated cuvette-holder compartment with temperature measuring probes and a NIR spectrometer to control the stimulated fluorescence emission of the nanoparticles\u2019 solutions. The temperature measurement compartment with the thermal-insulated cuvette holder was designed to maintain the solutions\u2019 temperature at a fixed value right before the moment of laser irradiation. To maintain the measurement setup at a fixed temperature before the irradiation we used a thermal stabilized system based on two Peltier cells with electronic temperature control. The temperatures of the ambient air and the temperature of the cuvette walls were continuously measured in order to make corrections about the temperature dissipation during the irradiation

Currency Crises During the Great Recession: Is This Time Different?

During the 2007-2009 financial crisis the foreign exchange market was characterized by large volatility and wide currency swings. In this paper we evaluate whether during the period of the Great Recession there has been a structural break in the relationship between fundamentals and exchange rates within an early-warning framework. This is done by extending the original data set by Kaminsky and Reinhart (1999) and including not only the most recent period, but also 17 new countries. Our analysis considers two variations of the original early-warning system. First, we propose two new methods to obtain the probability distribution of the early-warning indicator (conditional on the occurrence of a crisis) – one fully parametric and one based on a novel distribution-free semi-parametric approach. Second, we compare the original early-warning indicator with a core indicator that includes only “pseudo-financial variables” (domestic credit/GDP, the real exchange rate, international reserves and the real interest-rate differential) and we evaluate their performance not only for currency crises during the Great Recession, but also for the Asian Crisis. All tests make us conclude that “this time is different”, i.e. early-warning systems based on traditional macroeconomic variables have not only failed to forecast currency crises during the Great Recession, but have also significantly worsened with respect to the period of the Asian crisis.Early Warning Systems; Exchange Rates; Semi-parametric Meth- ods

Changes in co-existence mechanisms along a long-term soil chronosequence revealed by functional trait diversity

1. Functional trait diversity can reveal mechanisms of species co-existence in plant communities. Few studies have tested whether functional diversity for foliar traits related to resource use strategy increases or decreases with declining soil phosphorus (P) in forest communities. 2. We quantified tree basal area and four foliar functional traits (i.e. nitrogen (N), phosphorus (P), thickness and tissue density) for all woody species along the c. 120 kyr Franz Josef soil chronosequence in cool temperate rainforest, where strong shifts occur in light and soil nutrient availability (i.e. total soil P declines from 805 to 100 mg g–1). We combined the abundance and trait data in functional diversity indices to quantify trait convergence and divergence, in an effort to determine whether mechanisms of co-existence change with soil fertility. 3. Relationships between species trait means and total soil N and P were examined using multiple regression, with and without weighting of species abundances. We used Rao’s quadratic entropy to quantify functional diversity at the plot scale, then compared this with random expectation, using a null model that randomizes abundances across species within plots. Taxonomic diversity was measured using Simpson’s Diversity. Relationships between functional and taxonomic diversity and total soil P were examined using jackknife linear regression. 4. Leaf N and P declined and leaf thickness and density increased monotonically with declining total soil P along the sequence; these relationships were unaffected by abundance-weighting of species in the analyses. Inclusion of total soil N did not improve predictions of trait means. All measures of diversity calculated from presence/absence data were unrelated to total soil N and P. There was no evidence for a relationship between Rao values using quantitative abundances and total soil P. However, there was a strongly positive relationship between Rao, expressed relative to random expectation, and total soil P, indicating trait convergence of dominant species as soil P declined. 5. Synthesis: Our results demonstrate that at high fertility dominant species differ in resource use strategy, but as soil fertility declines over the long-term, dominant species increasingly converge on a resource-retentive strategy. This suggests that differentiation in resource use strategy is required for co-existence at high fertility but not in low fertility ecosystems

Biodegradation of Crude Oil by Penicillin sp and Mortierella sp. Isolated from Oil-Contaminated Soil in Auto Mechanic Workshops

Biodegrading capability of fungi isolated from oil-contaminated soil in three auto-mechanic workshops in Minna, Nigeria was determined by mycelial extension rate measurement method. Penicillium strain 8 I 0 IF, 8201 F and B202F and Mortierel/a strain B I 002F were identified as best crude oil (lagoma light) degraders compared to the other isolates such Aspergillus strain BI02F, BI04F and Bll9F, Fusarium strain 8501F and Trichoderma strain K602F and K561F. The optimum pH and temperature forB I 0 IF and B l 002F to degrade crude oil were 5.0 and 28 •c respectively. When complex nutrient such as maize bran was used as nitrogen source with crude oil in minimal salt liquid medium (MSLM) omitting NaN03, the optimum growth reached on 14tl' day of fermentation for both the isolates. BIOIF and BI002F were capable of using crude oil as both carbon and energy source in minimal salt medium. In view of their ability to utilize crude oil as carbon and nitrogen source, these organisms may be used in mitigating the pollution arising out of oil spills in the environmen

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Cadernos de SociomuseologiaNo intuito de divulgar e ampliar o seu circuito de vendas, a EMPRESA CIENTÍFICA MUSEOLOGIA L.DA., produtora dos BOMBONS MUSEU, aproveitando a oportunidade, apresenta aos seus consumidores, dois dos seus inúmeros produtos. Examine com o devido cuidado e faça a sua opção

Discurso histórico-panegírico de Santa Teresa de Jesús : en la inaguración de su primer convento en Ávila verificada en el día 24 de Agosto de 1562

Copia digital. Valladolid : Junta de Castilla y León. Consejería de Cultura y Turismo, 201